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KMID : 0360319790110010031
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Journal of Korean Cancer Research Association
1979 Volume.11 No. 1 p.31 ~ p.34
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이대일/Lee Dale
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Abstract
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Pinellia tumberg agglutinin was purified from saline extract by ammonium sulfate (60%) precipitation and successive DEAE Sepharose chromatography in 0.05M Tris HCL pH 8.0 with linear NaCl gradient. An active fraction was eluted in 0.1~0.25M NaCl and its agglutinating activity to L5178Y was 2,500 units/mg with high cytotoxicity to L5178Y in saline suspension but weak toxicity in Fischer´s medium.
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